Iron homeostasis alteration in transgenic tobacco overexpressing ferritin.
Identifieur interne : 000258 ( France/Analysis ); précédent : 000257; suivant : 000259Iron homeostasis alteration in transgenic tobacco overexpressing ferritin.
Auteurs : O. Van Wuytswinkel [France] ; G. Vansuyt ; N. Grignon ; P. Fourcroy ; J F BriatSource :
- The Plant journal : for cell and molecular biology [ 0960-7412 ] ; 1999.
Descripteurs français
- KwdFr :
- ADN complémentaire, FMN reductase, Fer (métabolisme), Ferritines (génétique), Ferritines (métabolisme), Feuilles de plante, Homéostasie, NADH, NADPH oxidoreductases (métabolisme), Protéines recombinantes (métabolisme), Racines de plante (enzymologie), Réaction de polymérisation en chaîne, Soja, Tabac (métabolisme), Végétaux génétiquement modifiés, Végétaux toxiques.
- MESH :
- enzymologie : Racines de plante.
- génétique : Ferritines.
- métabolisme : Fer, Ferritines, NADH, NADPH oxidoreductases, Protéines recombinantes, Tabac.
- ADN complémentaire, FMN reductase, Feuilles de plante, Homéostasie, Réaction de polymérisation en chaîne, Soja, Végétaux génétiquement modifiés, Végétaux toxiques.
English descriptors
- KwdEn :
- DNA, Complementary, FMN Reductase, Ferritins (genetics), Ferritins (metabolism), Homeostasis, Iron (metabolism), NADH, NADPH Oxidoreductases (metabolism), Plant Leaves, Plant Roots (enzymology), Plants, Genetically Modified, Plants, Toxic, Polymerase Chain Reaction, Recombinant Proteins (metabolism), Soybeans, Tobacco (metabolism).
- MESH :
- chemical , genetics : Ferritins.
- chemical , metabolism : Ferritins, Iron, NADH, NADPH Oxidoreductases, Recombinant Proteins.
- chemical : DNA, Complementary, FMN Reductase.
- enzymology : Plant Roots.
- metabolism : Tobacco.
- Homeostasis, Plant Leaves, Plants, Genetically Modified, Plants, Toxic, Polymerase Chain Reaction, Soybeans.
Abstract
Intracellular iron concentration requires tight control and is regulated both at the uptake and storage levels. Our knowledge of the role that the iron-storage protein ferritins play in plants is still very limited. Overexpression of this protein, either in the cytoplasm or the plastids of transgenic tobacco, was obtained by placing soybean ferritin cDNA cassettes under the control of the CAMV 35S promoter. The protein accumulated in 4- and 6-day-old seedlings and in leaves of 3-week-old plants but not in dry seeds or in 2-day-old seedlings, which is consistent with previous reports describing a post-transcriptional control of ferritin amounts during the germination process. Overaccumulated ferritin in leaves was correctly assembled as 24-mers. Transformants were more resistant to methylviologen toxicity, indicating that the transgenic ferritins were functional in vivo. Ferritin overaccumulation in transgenic tobacco leaves leads to an illegitimate iron sequestration. As a consequence, these transgenic plants behave as iron deficient and activate iron transport systems as revealed by an increase in root ferric reductase activity and in leaf iron content.
DOI: 10.1046/j.1365-313x.1999.00349.x
PubMed: 10069070
Affiliations:
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pubmed:10069070Le document en format XML
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Van Wuytswinkel</name><affiliation wicri:level="3"><nlm:affiliation>Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique (URA 2133), Institut National de la Recherche Agronomique et Ecole Nationale Supérieure d'Agronomie, Montpellier, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique (URA 2133), Institut National de la Recherche Agronomique et Ecole Nationale Supérieure d'Agronomie, Montpellier</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Vansuyt, G" sort="Vansuyt, G" uniqKey="Vansuyt G" first="G" last="Vansuyt">G. 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Fourcroy</name></author><author><name sortKey="Briat, J F" sort="Briat, J F" uniqKey="Briat J" first="J F" last="Briat">J F Briat</name></author></analytic><series><title level="j">The Plant journal : for cell and molecular biology</title><idno type="ISSN">0960-7412</idno><imprint><date when="1999" type="published">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Complementary</term><term>FMN Reductase</term><term>Ferritins (genetics)</term><term>Ferritins (metabolism)</term><term>Homeostasis</term><term>Iron (metabolism)</term><term>NADH, NADPH Oxidoreductases (metabolism)</term><term>Plant Leaves</term><term>Plant Roots (enzymology)</term><term>Plants, Genetically Modified</term><term>Plants, Toxic</term><term>Polymerase Chain Reaction</term><term>Recombinant Proteins (metabolism)</term><term>Soybeans</term><term>Tobacco (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire</term><term>FMN reductase</term><term>Fer (métabolisme)</term><term>Ferritines (génétique)</term><term>Ferritines (métabolisme)</term><term>Feuilles de plante</term><term>Homéostasie</term><term>NADH, NADPH oxidoreductases (métabolisme)</term><term>Protéines recombinantes (métabolisme)</term><term>Racines de plante (enzymologie)</term><term>Réaction de polymérisation en chaîne</term><term>Soja</term><term>Tabac (métabolisme)</term><term>Végétaux génétiquement modifiés</term><term>Végétaux toxiques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Ferritins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Ferritins</term><term>Iron</term><term>NADH, NADPH Oxidoreductases</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Complementary</term><term>FMN Reductase</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Ferritines</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Fer</term><term>Ferritines</term><term>NADH, NADPH oxidoreductases</term><term>Protéines recombinantes</term><term>Tabac</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Homeostasis</term><term>Plant Leaves</term><term>Plants, Genetically Modified</term><term>Plants, Toxic</term><term>Polymerase Chain Reaction</term><term>Soybeans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN complémentaire</term><term>FMN reductase</term><term>Feuilles de plante</term><term>Homéostasie</term><term>Réaction de polymérisation en chaîne</term><term>Soja</term><term>Végétaux génétiquement modifiés</term><term>Végétaux toxiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Intracellular iron concentration requires tight control and is regulated both at the uptake and storage levels. Our knowledge of the role that the iron-storage protein ferritins play in plants is still very limited. Overexpression of this protein, either in the cytoplasm or the plastids of transgenic tobacco, was obtained by placing soybean ferritin cDNA cassettes under the control of the CAMV 35S promoter. The protein accumulated in 4- and 6-day-old seedlings and in leaves of 3-week-old plants but not in dry seeds or in 2-day-old seedlings, which is consistent with previous reports describing a post-transcriptional control of ferritin amounts during the germination process. Overaccumulated ferritin in leaves was correctly assembled as 24-mers. Transformants were more resistant to methylviologen toxicity, indicating that the transgenic ferritins were functional in vivo. Ferritin overaccumulation in transgenic tobacco leaves leads to an illegitimate iron sequestration. As a consequence, these transgenic plants behave as iron deficient and activate iron transport systems as revealed by an increase in root ferric reductase activity and in leaf iron content.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li></region><settlement><li>Montpellier</li></settlement></list><tree><noCountry><name sortKey="Briat, J F" sort="Briat, J F" uniqKey="Briat J" first="J F" last="Briat">J F Briat</name><name sortKey="Fourcroy, P" sort="Fourcroy, P" uniqKey="Fourcroy P" first="P" last="Fourcroy">P. Fourcroy</name><name sortKey="Grignon, N" sort="Grignon, N" uniqKey="Grignon N" first="N" last="Grignon">N. Grignon</name><name sortKey="Vansuyt, G" sort="Vansuyt, G" uniqKey="Vansuyt G" first="G" last="Vansuyt">G. Vansuyt</name></noCountry><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Van Wuytswinkel, O" sort="Van Wuytswinkel, O" uniqKey="Van Wuytswinkel O" first="O" last="Van Wuytswinkel">O. Van Wuytswinkel</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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